Federer's shirt, jacket, socks, and signature headband all were adorned with the brand's red and white logo.
Federer's endorsements are estimated by Forbes to be worth a whopping US$65 million per year, with his long-running and high-profile partnership with Nike believed to rope in an eye-watering US$10 million annually.
"I felt very good from the start which is nice", Federer, who has now one 24 consecutive sets at the All England Club after blazing to the title a year ago without dropping one, said on court.
During his partnership with Nike Federer created the iconic "RF" logo, which is owned by the brand.
Federer, who wore a plain white bandana with two small red squares on the side while defeating Serbia's Dusan Lajovic 6-1 6-3 6-4 in the first round, said he was "excited" at being sponsored by the Japanese casual wear designer.
Stay Safe: Excessive Heat Warning in Effect Sunday and Monday
Monday will bring little relief with temperatures expected to hit a high of 98 degrees, according to the weather service. Sleeping will be extremely hard without air conditioning as heat from the pavement will be ruthless, USA Today reports.
Uniqlo said Federer, who has won a men's record of 20 Grand Slam titles, will represent the brand at all tennis tournaments throughout the year.
The 36-year-old left-handed Spaniard had equalled the mark set by Roger Federer at Roland Garros.
"After a while without playing on grass it's very important to start with a straight victory", he said.
"In previous years we have never shown the football, whether it is the European Championships or the World Cup, and there will be no change to that", Richard Lewis, AELTC CEO, said. "They are mine. The good thing is it's not theirs forever".
The new relationship gives Uniqlo another shot at exploiting one of the sport's all-time greats.
"Uniqlo will help Mr Federer continue taking tennis to new places, while exploring innovations in a number of areas including technology and design with him". Federer is still representing the Nike swoosh on his shoes.